The research object of Transcriptome sequencing is the sum of all RNA that can be transcribed by a specific cell under a certain functional state. Current sequencing technology mainly targets mRNA. Transcriptome sequencing technology can obtain the issue of changes in gene expression levels, discovery of rare transcripts, precise identification of alternative splicing sites, and gene fusions, provide the most comprehensive transcriptome information. Through sequencing, we can obtain almost all transcript sequence information of a specific tissue or organ of a specie under a certain state. which is widely used in species genetic development, improvement, and molecular breeding research.